Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10773/37451
Título: | Optimization of phycobiliprotein pigments extraction from red algae Gracilaria gracilis for substitution of synthetic food colorants |
Autor: | Pereira, Tatiana Barroso, Sónia Mendes, Susana Amaral, Renata A. Dias, Juliana R. Baptista, Teresa Saraiva, Jorge A. Alves, Nuno M. Gil, Maria M. |
Palavras-chave: | Natural pigments Phycobiliproteins Gracilaria gracilis Response surface methodology Extraction optimization |
Data: | 15-Ago-2020 |
Editora: | Elsevier |
Resumo: | The extraction of phycobiliprotein (PBP) pigments from red algae Gracilaria gracilis was optimized using maceration, ultrasound-assisted extraction (ultrasonic water bath and ultrasonic probe), high pressure-assisted extraction, and freeze-thaw. The experimental conditions, namely homogenization time (t1), buffer concentration (C), treatment time (t2), biomass: buffer ratio (R), and pressure (P), were optimized using Response Surface Methodology (RSM). The yield of phycoerythrin (PE) extracted, determined spectroscopically, was used as the response variable. Maceration was the most efficient extraction method yielding 3.6 mg PE/g biomass under the optimal conditions (t1 = t2 = 10 min; C = 0.1 M; R = 1:50). Scanning Electron Microscopy (SEM) analysis of the biomass before and after the cell disruption treatments revealed a more efficient cell wall rupture with maceration. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/37451 |
DOI: | 10.1016/j.foodchem.2020.126688 |
ISSN: | 0308-8146 |
Aparece nas coleções: | DQ - Artigos QOPNA - Artigos REQUIMTE - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Optimization of phycobiliprotein pigments extraction from red algae.pdf | 1.53 MB | Adobe PDF | Ver/Abrir |
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.