Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/37451
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DC Field | Value | Language |
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dc.contributor.author | Pereira, Tatiana | pt_PT |
dc.contributor.author | Barroso, Sónia | pt_PT |
dc.contributor.author | Mendes, Susana | pt_PT |
dc.contributor.author | Amaral, Renata A. | pt_PT |
dc.contributor.author | Dias, Juliana R. | pt_PT |
dc.contributor.author | Baptista, Teresa | pt_PT |
dc.contributor.author | Saraiva, Jorge A. | pt_PT |
dc.contributor.author | Alves, Nuno M. | pt_PT |
dc.contributor.author | Gil, Maria M. | pt_PT |
dc.date.accessioned | 2023-04-28T11:48:16Z | - |
dc.date.available | 2023-04-28T11:48:16Z | - |
dc.date.issued | 2020-08-15 | - |
dc.identifier.issn | 0308-8146 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/37451 | - |
dc.description.abstract | The extraction of phycobiliprotein (PBP) pigments from red algae Gracilaria gracilis was optimized using maceration, ultrasound-assisted extraction (ultrasonic water bath and ultrasonic probe), high pressure-assisted extraction, and freeze-thaw. The experimental conditions, namely homogenization time (t1), buffer concentration (C), treatment time (t2), biomass: buffer ratio (R), and pressure (P), were optimized using Response Surface Methodology (RSM). The yield of phycoerythrin (PE) extracted, determined spectroscopically, was used as the response variable. Maceration was the most efficient extraction method yielding 3.6 mg PE/g biomass under the optimal conditions (t1 = t2 = 10 min; C = 0.1 M; R = 1:50). Scanning Electron Microscopy (SEM) analysis of the biomass before and after the cell disruption treatments revealed a more efficient cell wall rupture with maceration. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMAR%2F04292%2F2019/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04044%2F2019/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00062%2F2019/PT | pt_PT |
dc.relation | ROTEIRO/0328/2013 | pt_PT |
dc.relation | 022158 | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT | pt_PT |
dc.relation | ENTRO-01-0145-FEDER-000018 | pt_PT |
dc.relation | CENTRO-01-0145-FEDER-000014 | pt_PT |
dc.relation | 16-02-01-FMP-84 | pt_PT |
dc.rights | openAccess | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
dc.subject | Natural pigments | pt_PT |
dc.subject | Phycobiliproteins | pt_PT |
dc.subject | Gracilaria gracilis | pt_PT |
dc.subject | Response surface methodology | pt_PT |
dc.subject | Extraction optimization | pt_PT |
dc.title | Optimization of phycobiliprotein pigments extraction from red algae Gracilaria gracilis for substitution of synthetic food colorants | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.title | Food chemistry | pt_PT |
degois.publication.volume | 321 | pt_PT |
dc.identifier.doi | 10.1016/j.foodchem.2020.126688 | pt_PT |
dc.identifier.articlenumber | 126688 | pt_PT |
Appears in Collections: | DQ - Artigos QOPNA - Artigos REQUIMTE - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Optimization of phycobiliprotein pigments extraction from red algae.pdf | 1.53 MB | Adobe PDF | View/Open |
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