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Title: Optimization of phycobiliprotein pigments extraction from red algae Gracilaria gracilis for substitution of synthetic food colorants
Author: Pereira, Tatiana
Barroso, Sónia
Mendes, Susana
Amaral, Renata A.
Dias, Juliana R.
Baptista, Teresa
Saraiva, Jorge A.
Alves, Nuno M.
Gil, Maria M.
Keywords: Natural pigments
Gracilaria gracilis
Response surface methodology
Extraction optimization
Issue Date: 15-Aug-2020
Publisher: Elsevier
Abstract: The extraction of phycobiliprotein (PBP) pigments from red algae Gracilaria gracilis was optimized using maceration, ultrasound-assisted extraction (ultrasonic water bath and ultrasonic probe), high pressure-assisted extraction, and freeze-thaw. The experimental conditions, namely homogenization time (t1), buffer concentration (C), treatment time (t2), biomass: buffer ratio (R), and pressure (P), were optimized using Response Surface Methodology (RSM). The yield of phycoerythrin (PE) extracted, determined spectroscopically, was used as the response variable. Maceration was the most efficient extraction method yielding 3.6 mg PE/g biomass under the optimal conditions (t1 = t2 = 10 min; C = 0.1 M; R = 1:50). Scanning Electron Microscopy (SEM) analysis of the biomass before and after the cell disruption treatments revealed a more efficient cell wall rupture with maceration.
Peer review: yes
DOI: 10.1016/j.foodchem.2020.126688
ISSN: 0308-8146
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