Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10773/36307
Título: | Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems |
Autor: | Ventura, Sónia Patrícia Marques Barros, Rafael Lemos Freire de Barbosa, José Murillo de Pinho Soares, Cleide Mara Faria Lima, Álvaro Silva Coutinho, João Araújo Pereira |
Data: | 3-Jan-2012 |
Editora: | Royal Society of Chemistry |
Resumo: | The ability of ionic liquid-based aqueous two-phase systems (ATPS) to purify lipase produced by fermentation is here evaluated and compared against conventional PEG-based ATPS systems. Four ionic liquids, chosen after screening of a larger number of ionic liquids are evaluated, with the maximum purification and higher recovery being obtained for the systems based on [C8mim]Cl. It is shown that IL-based ATPS have a performance superior to PEG-based ATPS for the purification of this enzyme. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/36307 |
DOI: | 10.1039/c2gc16428k |
ISSN: | 1463-9262 |
Aparece nas coleções: | CICECO - Artigos DQ - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
L.pdf | 1.15 MB | Adobe PDF |
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