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Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/36307
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dc.contributor.authorVentura, Sónia Patrícia Marquespt_PT
dc.contributor.authorBarros, Rafael Lemos Freire dept_PT
dc.contributor.authorBarbosa, José Murillo de Pinhopt_PT
dc.contributor.authorSoares, Cleide Mara Fariapt_PT
dc.contributor.authorLima, Álvaro Silvapt_PT
dc.contributor.authorCoutinho, João Araújo Pereirapt_PT
dc.date.accessioned2023-02-14T11:40:58Z-
dc.date.available2023-02-14T11:40:58Z-
dc.date.issued2012-01-03-
dc.identifier.issn1463-9262pt_PT
dc.identifier.urihttp://hdl.handle.net/10773/36307-
dc.description.abstractThe ability of ionic liquid-based aqueous two-phase systems (ATPS) to purify lipase produced by fermentation is here evaluated and compared against conventional PEG-based ATPS systems. Four ionic liquids, chosen after screening of a larger number of ionic liquids are evaluated, with the maximum purification and higher recovery being obtained for the systems based on [C8mim]Cl. It is shown that IL-based ATPS have a performance superior to PEG-based ATPS for the purification of this enzyme.pt_PT
dc.description.sponsorshipThe authors are grateful for financial support from Fundação para a Ciência e a Tecnologia for the Ph.D. grant SFRH/BD/37830/2007 of S.P.M. Ventura. Moreover, the authors are grateful for the financial support from CAPES for the Ph.D. grant 551223/2010-3 of José M. P. Barbosa and grant PIBIC from FAPITEC for the grant of Rafael L. F. de Barros.pt_PT
dc.language.isoengpt_PT
dc.publisherRoyal Society of Chemistrypt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F37830%2F2007/PTpt_PT
dc.rightsrestrictedAccesspt_PT
dc.titleProduction and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systemspt_PT
dc.typearticlept_PT
dc.description.versionpublishedpt_PT
dc.peerreviewedyespt_PT
degois.publication.firstPage734pt_PT
degois.publication.issue3pt_PT
degois.publication.lastPage740pt_PT
degois.publication.titleGreen Chemistrypt_PT
degois.publication.volume14pt_PT
dc.identifier.doi10.1039/c2gc16428kpt_PT
dc.identifier.essn1463-9270pt_PT
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