Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/23213
Title: | Evaluation of the anthropogenic input of caffeine in surface waters of the north and center of portugal by ELISA |
Author: | Silva, Carla Patrícia Lima, Diana L. D. Schneider, Rudolf J. Otero, Marta Esteves, Valdemar I. |
Keywords: | Anthropogenic markers Caffeine ELISA Surface water |
Issue Date: | 2014 |
Publisher: | Elsevier |
Abstract: | This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1-100??gL-1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between <LOD and 15??gL-1. Results correlate well with those obtained by LC-MS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/23213 |
DOI: | 10.1016/j.scitotenv.2014.01.120 |
ISSN: | 0048-9697 |
Appears in Collections: | CESAM - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Silva et al. - 2014 - Evaluation of the anthropogenic input of caffeine .pdf | 346.22 kB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.