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http://hdl.handle.net/10773/23213
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DC Field | Value | Language |
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dc.contributor.author | Silva, Carla Patrícia | pt |
dc.contributor.author | Lima, Diana L. D. | pt |
dc.contributor.author | Schneider, Rudolf J. | pt |
dc.contributor.author | Otero, Marta | pt |
dc.contributor.author | Esteves, Valdemar I. | pt |
dc.date.accessioned | 2018-05-23T11:22:19Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0048-9697 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/23213 | - |
dc.description.abstract | This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1-100??gL-1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between <LOD and 15??gL-1. Results correlate well with those obtained by LC-MS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters. | pt |
dc.language.iso | eng | pt |
dc.publisher | Elsevier | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/132951/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F74430%2F2010/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F80315%2F2011/PT | pt |
dc.relation | RYC-2010-05634 | pt |
dc.rights | restrictedAccess | por |
dc.subject | Anthropogenic markers | pt |
dc.subject | Caffeine | pt |
dc.subject | ELISA | pt |
dc.subject | Surface water | pt |
dc.title | Evaluation of the anthropogenic input of caffeine in surface waters of the north and center of portugal by ELISA | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 227 | pt |
degois.publication.issue | 1 | |
degois.publication.lastPage | 232 | pt |
degois.publication.title | Science of the Total Environment | pt |
degois.publication.volume | 479-480 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.doi | 10.1016/j.scitotenv.2014.01.120 | pt |
Appears in Collections: | CESAM - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Silva et al. - 2014 - Evaluation of the anthropogenic input of caffeine .pdf | 346.22 kB | Adobe PDF |
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