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http://hdl.handle.net/10773/23213
Título: | Evaluation of the anthropogenic input of caffeine in surface waters of the north and center of portugal by ELISA |
Autor: | Silva, Carla Patrícia Lima, Diana L. D. Schneider, Rudolf J. Otero, Marta Esteves, Valdemar I. |
Palavras-chave: | Anthropogenic markers Caffeine ELISA Surface water |
Data: | 2014 |
Editora: | Elsevier |
Resumo: | This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1-100??gL-1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between <LOD and 15??gL-1. Results correlate well with those obtained by LC-MS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/23213 |
DOI: | 10.1016/j.scitotenv.2014.01.120 |
ISSN: | 0048-9697 |
Aparece nas coleções: | CESAM - Artigos DQ - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Silva et al. - 2014 - Evaluation of the anthropogenic input of caffeine .pdf | 346.22 kB | Adobe PDF |
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