Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/36307| Title: | Production and purification of an extracellular lipolytic enzyme using ionic liquid-based aqueous two-phase systems |
| Author: | Ventura, Sónia Patrícia Marques Barros, Rafael Lemos Freire de Barbosa, José Murillo de Pinho Soares, Cleide Mara Faria Lima, Álvaro Silva Coutinho, João Araújo Pereira |
| Issue Date: | 3-Jan-2012 |
| Publisher: | Royal Society of Chemistry |
| Abstract: | The ability of ionic liquid-based aqueous two-phase systems (ATPS) to purify lipase produced by fermentation is here evaluated and compared against conventional PEG-based ATPS systems. Four ionic liquids, chosen after screening of a larger number of ionic liquids are evaluated, with the maximum purification and higher recovery being obtained for the systems based on [C8mim]Cl. It is shown that IL-based ATPS have a performance superior to PEG-based ATPS for the purification of this enzyme. |
| Peer review: | yes |
| URI: | http://hdl.handle.net/10773/36307 |
| DOI: | 10.1039/c2gc16428k |
| ISSN: | 1463-9262 |
| Appears in Collections: | CICECO - Artigos DQ - Artigos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| L.pdf | 1.15 MB | Adobe PDF |  |
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