Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/29519
Title: Chronic exercise training attenuates prostate cancer-induced molecular remodelling in the testis
Author: Matos, Bárbara
Patrício, Daniela
Henriques, Magda C.
Freitas, Maria J.
Vitorino, Rui
Duarte, Iola F.
Howl, John
Oliveira, Paula
Seixas, Fernanda
Duarte, José A.
Ferreira, Rita
Fardilha, Margarida
Keywords: Prostate cancer
Exercise training
Testicular function
Oxidative stress
DNA repair
Metabolism
Issue Date: 19-Oct-2020
Publisher: Springer
Abstract: Purpose: Prostate cancer is a major cause of cancer-related death in males worldwide and, in addition to impairing prostate function, also causes testicular adaptations. In this study, we aim to investigate the preventive effect of exercise training on PCa-induced testicular dysfunction. Methods: As a model, we used fifty Wistar Unilever male rats, randomly divided in four experimental groups. Prostate cancer was chemically and hormonally induced in two groups of animals (PCa groups). One control group and one PCa group was submitted to moderate intensity treadmill exercise training. Fifty weeks after the start of the training the animals were sacrificed and sperm, prostate, testis and serum were collected and analyzed. Sperm concentration and morphology, and testosterone serum levels were determined. In addition, histological analyses of the testes were performed, and testis proteomes and metabolomes were characterized. Results: We found that prostate cancer negatively affected testicular function, manifested as an arrest of spermatogenesis. Oxidative stress-induced DNA damage, arising from reduced testis blood flow, may also contribute to apoptosis of germ cells and consequential spermatogenic impairment. Decreased utilization of the glycolytic pathway, increased metabolism of ketone bodies and the accumulation of branched chain amino acids were also evident in the PCa animals. Conversely, we found that the treadmill training regimen activated DNA repair mechanisms and counteracted several metabolic alterations caused by PCa without impact on oxidative stress. Conclusions: These findings confirm a negative impact of prostate cancer on testis function and suggest a beneficial role for exercise training in the prevention of prostate cancer-induced testis dysfunction.
Peer review: yes
URI: http://hdl.handle.net/10773/29519
DOI: 10.1007/s13402-020-00567-9
ISSN: 2211-3428
Appears in Collections:CICECO - Artigos
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