Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/28219
Title: | Purification, crystallization and preliminary X-ray diffraction analysis of the seryl-tRNA synthetase from Candida albicans |
Author: | Rocha, Rita Pereira, Pedro José Barbosa Santos, Manuel A. S. Macedo-Ribeiro, Sandra |
Issue Date: | 1-Jan-2011 |
Publisher: | International Union of Crystallography |
Abstract: | The seryl-tRNA synthetase (SerRS) from Candida albicans exists naturally as two isoforms resulting from ambiguity in the natural genetic code. Both enzymes were crystallized by the sitting-drop vapour-diffusion method using 3.2-3.4 M ammonium sulfate as precipitant. The crystals belonged to the hexagonal space group P6(1)22 and contained one monomer per asymmetric unit, despite the synthetase existing as a homodimer (with a molecular weight of ∼116 kDa) in solution. Diffraction data were collected to 2.0 Å resolution at a synchrotron source and the crystal structures of unliganded SerRS and of its complexes with ATP and with a seryl-adenylate analogue were solved by molecular replacement. The structure of C. albicans SerRS represents the first reported structure of a eukaryotic cytoplasmic SerRS. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/28219 |
DOI: | 10.1107/S1744309110048542 |
ISSN: | 1744-3091 |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Rocha et al. - 2011 - Purification, crystallization and preliminary X-ra.pdf | 239.45 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.