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http://hdl.handle.net/10773/28219
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DC Field | Value | Language |
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dc.contributor.author | Rocha, Rita | pt_PT |
dc.contributor.author | Pereira, Pedro José Barbosa | pt_PT |
dc.contributor.author | Santos, Manuel A. S. | pt_PT |
dc.contributor.author | Macedo-Ribeiro, Sandra | pt_PT |
dc.date.accessioned | 2020-04-08T10:26:54Z | - |
dc.date.available | 2020-04-08T10:26:54Z | - |
dc.date.issued | 2011-01-01 | - |
dc.identifier.issn | 1744-3091 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/28219 | - |
dc.description.abstract | The seryl-tRNA synthetase (SerRS) from Candida albicans exists naturally as two isoforms resulting from ambiguity in the natural genetic code. Both enzymes were crystallized by the sitting-drop vapour-diffusion method using 3.2-3.4 M ammonium sulfate as precipitant. The crystals belonged to the hexagonal space group P6(1)22 and contained one monomer per asymmetric unit, despite the synthetase existing as a homodimer (with a molecular weight of ∼116 kDa) in solution. Diffraction data were collected to 2.0 Å resolution at a synchrotron source and the crystal structures of unliganded SerRS and of its complexes with ATP and with a seryl-adenylate analogue were solved by molecular replacement. The structure of C. albicans SerRS represents the first reported structure of a eukaryotic cytoplasmic SerRS. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | International Union of Crystallography | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/70634/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/64745/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/98850/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/99826/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F15233%2F2004/PT | pt_PT |
dc.rights | openAccess | pt_PT |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.title | Purification, crystallization and preliminary X-ray diffraction analysis of the seryl-tRNA synthetase from Candida albicans | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.firstPage | 153 | pt_PT |
degois.publication.issue | Pt 1 | pt_PT |
degois.publication.lastPage | 156 | pt_PT |
degois.publication.title | Acta Crystallographica Section F: Structural Biology and Crystallization Communications | pt_PT |
degois.publication.volume | F67 | pt_PT |
dc.identifier.doi | 10.1107/S1744309110048542 | pt_PT |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Rocha et al. - 2011 - Purification, crystallization and preliminary X-ra.pdf | 239.45 kB | Adobe PDF | View/Open |
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