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|Title:||Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants|
|Author:||Vicente, Filipa A.|
Lario, Luciana D.
Pessoa, Adalberto, Jr.
Ventura, Sonia P. M.
|Keywords:||COMOSUS L. MERRYL|
|Publisher:||ELSEVIER SCI LTD|
|Abstract:||Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved.|
|Appears in Collections:||CICECO - Artigos|
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|Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants_10.1016j.procbio.2016.01.004.pdf||1.04 MB||Adobe PDF|
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