Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/20166
Title: Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants
Author: Vicente, Filipa A.
Lario, Luciana D.
Pessoa, Adalberto, Jr.
Ventura, Sonia P. M.
Keywords: COMOSUS L. MERRYL
ANANAS-COMOSUS
EXTRACT BROMELAIN
REVERSED MICELLES
BIPHASIC SYSTEMS
PEEL WASTE
PURIFICATION
OPTIMIZATION
SEPARATION
PROTEINS
Issue Date: 2016
Publisher: ELSEVIER SCI LTD
Abstract: Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved.
Peer review: yes
URI: http://hdl.handle.net/10773/20166
DOI: 10.1016/j.procbio.2016.01.004
ISSN: 1359-5113
Publisher Version: 10.1016/j.procbio.2016.01.004
Appears in Collections:CICECO - Artigos



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