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http://hdl.handle.net/10773/20166
Título: | Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants |
Autor: | Vicente, Filipa A. Lario, Luciana D. Pessoa Jr., Adalberto Ventura, Sónia P. M. |
Palavras-chave: | Bromelain Aqueous micellar two-phase system Triton X-114 Ionic liquids Co-surfactant Purification |
Data: | 2016 |
Editora: | Elsevier |
Resumo: | Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/20166 |
DOI: | 10.1016/j.procbio.2016.01.004 |
ISSN: | 1359-5113 |
Versão do Editor: | 10.1016/j.procbio.2016.01.004 |
Aparece nas coleções: | CICECO - Artigos DQ - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants_10.1016j.procbio.2016.01.004.pdf | 1.04 MB | Adobe PDF |
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