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http://hdl.handle.net/10773/20166
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DC Field | Value | Language |
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dc.contributor.author | Vicente, Filipa A. | pt |
dc.contributor.author | Lario, Luciana D. | pt |
dc.contributor.author | Pessoa Jr., Adalberto | pt |
dc.contributor.author | Ventura, Sónia P. M. | pt |
dc.date.accessioned | 2017-12-07T19:37:44Z | - |
dc.date.issued | 2016 | pt |
dc.identifier.issn | 1359-5113 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/20166 | - |
dc.description.abstract | Pineapple processing industries produce a large amount of residues; for example, the pineapple stem, which is rich in bromelain. Bromelain is a group of cysteine proteinases that have major importance in the pharmaceutical field due to, among others, their anti-inflammatory, antithrombotic, and fibrinolytic activities. Since Brazil is one of the largest producers of pineapples in the world, the development of an ecofriendly and cost-effective process to extract and purify bromelain from food/fruit residues would be significant. In this study, aqueous micellar two-phase systems composed of ionic liquids as co-surfactants were evaluated in the extraction of bromelain from the pineapple stem crude extract. The main results showed that bromelain partitions preferentially toward the micelle-poor phase, with enzyme recoveries above 90% for the studied systems. Moreover, a stabilizing effect of the ionic liquids toward the enzyme was observed. (C) 2016 Elsevier Ltd. All rights reserved. | pt |
dc.language.iso | eng | pt |
dc.publisher | Elsevier | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/132936/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F79263%2F2011/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147332/PT | pt |
dc.rights | restrictedAccess | por |
dc.subject | Bromelain | pt |
dc.subject | Aqueous micellar two-phase system | pt |
dc.subject | Triton X-114 | pt |
dc.subject | Ionic liquids | pt |
dc.subject | Co-surfactant | pt |
dc.subject | Purification | pt |
dc.title | Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
degois.publication.firstPage | 528 | pt |
degois.publication.issue | 4 | pt_PT |
degois.publication.lastPage | 534 | pt |
degois.publication.title | Process Biochemistry | pt_PT |
degois.publication.volume | 51 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.relation.publisherversion | 10.1016/j.procbio.2016.01.004 | pt |
dc.identifier.doi | 10.1016/j.procbio.2016.01.004 | pt |
dc.identifier.essn | 1873-3298 | pt_PT |
Appears in Collections: | CICECO - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Recovery of bromelain from pineapple stem residues using aqueous micellar two-phase systems with ionic liquids as co-surfactants_10.1016j.procbio.2016.01.004.pdf | 1.04 MB | Adobe PDF |
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