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http://hdl.handle.net/10773/37513
Title: | Structure, rheology, and copper-complexation of a hyaluronan-like exopolysaccharide from Vibrio |
Author: | Martin-Pastor, Manuel Ferreira, Andreia S. Moppert, Xavier Nunes, Cláudia Coimbra, Manuel A. Reis, Rui L. Guezennec, Jean Novoa-Carballal, Ramon |
Keywords: | Exopolysaccharide Hyaluronic-like NMR spectroscopy Physicochemical properties Bacterial mats Vibrio sp |
Issue Date: | 15-Oct-2019 |
Publisher: | Elsevier |
Abstract: | MO245 exopolysaccharide (EPS) was produced in laboratory conditions from Vibrio genus microorganism isolated from bacterial mats found in Moorea Island. Its structure consists of a linear tetrasaccharide repeating unit →4)-β-D-GlcpA-(1→4)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→4)-β-D-GlcpA-(1→ containing covalently-linked 5% of glucose, galactose, and rhamnose, determined by methylation analyses and NMR spectroscopy. The molecular weight, radius of gyration (Rg) and intrinsic viscosity, [η], determined by gel permeation chromatography with light scattering and viscosity detection, were 513 ± 4 kDa (PDI, 1.42 ± 0.01), 6.7 ± 0.3 dl/g and 56 ± 0.3 nm respectively. The chelation of the EPS with copper divalent ions leads to the instantaneous formation of gels. The structural similitude proposed, based in an equal ratio of GlcA to N-acetylated sugars and in the same type of glyosidic linkages present in the repeating unit (alternated 1→3 and 1→4 linkages), is translated into analogous physicochemical properties: MO245 EPS is a flexible polyelectrolyte, with scaling exponents similar to that described for HA. This similitude opens opportunities in future drug delivery, tissue engineering, and cosmetic applications. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/37513 |
DOI: | 10.1016/j.carbpol.2019.114999 |
ISSN: | 0144-8617 |
Appears in Collections: | CICECO - Artigos DQ - Artigos QOPNA - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Structure, rheology, and copper-complexation of a.pdf | 1.4 MB | Adobe PDF | View/Open |
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