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http://hdl.handle.net/10773/37318
Title: | Innovative hurdle system towards Listeria monocytogenes inactivation in a fermented meat sausage model - high pressure processing assisted by bacteriophage P100 and bacteriocinogenic Pediococcus acidilactici |
Author: | Komora, Norton Maciel, Cláudia Amaral, Renata A. Fernandes, Rui Castro, Sónia Marília Saraiva, Jorge A. Teixeira, Paula |
Keywords: | Listeria monocytogenes High hydrostatic pressure Biocontrol approaches Bacteriophage Pediococcus acidilactici Pediocin PA-1 |
Issue Date: | Oct-2021 |
Publisher: | Elsevier |
Abstract: | Consumers' quest for healthier, locally produced foods, renders the demand for these products increasingly prominent. The purpose of the present work was to evaluate the impact of a non-thermal multi-hurdle approach, which associated mild high hydrostatic pressure (HHP, 300 MPa), the bacteriophage Listex™ P100, and the pediocin PA-1 producing Pediococcus acidilactici HA 6111-2, as a novel minimal processing towards Listeria monocytogenes eradication in Alheira (a traditional fermented meat sausage from Northern Portugal). The combination of the three hurdles achieved the USDA-FSIS 5 log reduction (in accordance with the standard guidelines for ready-to-eat foods), being the only treatment to elicit the absence of L. monocytogenes immediately following processing (p < 0.05). The pair association of HHP with Listex™ P100 was unable to eliminate L. monocytogenes, whilst in the HHP-pediocin PA-1 producing P. acidilactici treated samples the eradication was delayed when compared to the three hurdles combination. In addition to the listericidal effect of the HHP-phage-lactic acid bacterium treatment, no significant differences (p > 0.05) in the pH values were observed, and the semi-quantification of the in situ biosynthesized pediocin PA-1 was documented for the first time in a fermented meat sausage model. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/37318 |
DOI: | 10.1016/j.foodres.2021.110628 |
ISSN: | 0963-9969 |
Appears in Collections: | DQ - Artigos REQUIMTE - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Innovative hurdle system towards Listeria monocytogenes inactivation.pdf | 3.89 MB | Adobe PDF | View/Open |
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