Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/37280
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Maciel, Cláudia | pt_PT |
dc.contributor.author | Campos, Ana | pt_PT |
dc.contributor.author | Komora, Norton | pt_PT |
dc.contributor.author | Pinto, Carlos A. | pt_PT |
dc.contributor.author | Fernandes, Rui | pt_PT |
dc.contributor.author | Saraiva, Jorge A. | pt_PT |
dc.contributor.author | Teixeira, Paula | pt_PT |
dc.date.accessioned | 2023-04-21T13:59:52Z | - |
dc.date.available | 2023-04-21T13:59:52Z | - |
dc.date.issued | 2021-11 | - |
dc.identifier.issn | 0023-6438 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/37280 | - |
dc.description.abstract | This work consisted in the first comprehensive study in which the potential to exploit the Salmonella lytic bacteriophages cocktail, Salmonelex™, in association with high hydrostatic pressure (HHP) towards potential application in egg matrices decontamination was evaluated. The impact of HHP (200–600 MPa) on the bacteriophages' viability pointed out a stability in the range of 200–400 MPa. From 400 MPa onwards, the inactivation was potentiated by an increase in the pressure magnitude, being matrix dependent. Salmonelex™ possessed a prominent baroresistance, requiring 600 MPa to completely lose its infectivity. Egg yolk presented the highest baroprotective effect, followed by whole egg and egg white. Transmission electron microscopy unveiled that 500 and 600 MPa elicited a detrimental impact on the bacteriophages’ structural integrity. It was noteworthy the barotolerance (200–300 MPa) of Salmonelex™, previously exposed to different pH conditions (5.0–9.0), which proved not to undermine its infectivity. Regarding the influence of ovalbumin, lysozyme, l-α-phosphatidylcholine, palmitic and oleic acids on the mild HHP-induced inactivation of Salmonelex™, a baroprotective effect was observed, particularly conferred by those compounds comprising egg yolk. The promising results highlighted the feasibility of utilizing Salmonelex™ as an adjuvant to mild HHP processing of egg matrices. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50016%2F2020/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F104016%2F2014/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F137036%2F2018/PT | pt_PT |
dc.rights | openAccess | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
dc.subject | High hydrostatic pressure (HHP) | pt_PT |
dc.subject | Salmonella | pt_PT |
dc.subject | Bacteriophage SalmonelexTM | pt_PT |
dc.subject | Egg | pt_PT |
dc.subject | Matrix protection | pt_PT |
dc.title | Impact of high hydrostatic pressure on the stability of lytic bacteriophages cocktail Salmonelex™ towards potential application on Salmonella inactivation | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.title | LWT | pt_PT |
degois.publication.volume | 151 | pt_PT |
dc.identifier.doi | 10.1016/j.lwt.2021.112108 | pt_PT |
dc.identifier.articlenumber | 112108 | pt_PT |
Appears in Collections: | DQ - Artigos REQUIMTE - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Impact of high hydrostatic pressure on the stability of lytic bacteriophage cocktail.pdf | 2.05 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.