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http://hdl.handle.net/10773/36839
Title: | Autolytic changes involving proteolytic enzymes on Atlantic salmon (Salmo salar) preserved by hyperbaric storage |
Author: | Fidalgo, Liliana G. Delgadillo, Ivonne Saraiva, Jorge A. |
Keywords: | Hyperbaric storage Salmo salar Lysosomal enzymes Calpains Myofibrillar fragmentation index |
Issue Date: | Jan-2020 |
Publisher: | Elsevier |
Abstract: | he effect of hyperbaric storage (HS, 50–75 MPa at 10–37 °C) on proteolytic enzymes and muscle proteins of Atlantic salmon (Salmo salar) was assessed and compared to atmospheric pressure (AP, 0.1 MPa) at the same storage temperature and refrigeration (AP, 5 °C). Generally, activities of acid phosphatase, cathepsin B and D, and calpains decreased when compared to fresh salmon, with a more’ pronounced effect of storage temperature of 37 °C in HS/AP samples. However, activity recovery was observed for some enzymes, as the case of cathepsins B and D, and calpains, whose showed an increase of residual activity for samples stored at 60 MPa/10 °C and 75 MPa/25 °C after 50 and 25 d, respectively. A pronounced increase of myofibrillar fragmentation index (MFI) was observed at 75 MPa (25/37 °C) after 10 d (3.2-/4.3-fold, respectively). Otherwise, at 60 MPa/10 °C, a decrease of MFI values was observed after 50 d of storage. For sarcoplasmic proteins, no effect was observed at 60 MPa/10 °C during 30 d of storage, with a slight increase after 50 d. At 75 MPa/25 °C, a decrease of sarcoplasmic proteins content (46%) was obtained after 10 d with no further changes during the 25 d of storage. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/36839 |
DOI: | 10.1016/j.lwt.2019.108755 |
ISSN: | 0023-6438 |
Appears in Collections: | DQ - Artigos REQUIMTE - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Autolytic changes involving proteolytic enzymes on Atlantic salmon.pdf | 1.85 MB | Adobe PDF | View/Open |
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