Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/35645
Title: | Endo- and exometabolome crosstalk in mesenchymal stem cells undergoing osteogenic differentiation |
Author: | Bispo, Daniela S. C. Michálková, Lenka Correia, Marlene Jesus, Catarina S. H. Duarte, Iola F. Goodfellow, Brian J. Oliveira, Mariana B. Mano, João F. Gil, Ana M. |
Keywords: | Mesenchymal stem cells Osteogenic differentiation Osteogenesis NMR spectroscopy Metabolomics Lipidomics Endometabolome Exometabolome |
Issue Date: | 7-Apr-2022 |
Publisher: | MDPI |
Abstract: | This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosphatidylcholine, phosphatidylethanolamine, sphingomyelins, and plasmalogens), and mono- and triglycerides to be followed. Lipid changes occurred almost exclusively between days 1 and 7, followed by a tendency for lipidome stabilization after day 7. On average, phospholipids and longer and more unsaturated fatty acids increased up to day 7, probably related to plasma membrane fluidity. Articulation of lipidome changes with previously reported polar endometabolome profiling and with exometabolome changes reported here in the same cells, enabled important correlations to be established during hAMSC osteogenic differentiation. Our results supported hypotheses related to the dynamics of membrane remodelling, anti-oxidative mechanisms, protein synthesis, and energy metabolism. Importantly, the observation of specific up-taken or excreted metabolites paves the way for the identification of potential osteoinductive metabolites useful for optimized osteogenic protocols. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/35645 |
DOI: | 10.3390/cells11081257 |
Publisher Version: | https://www.mdpi.com/2073-4409/11/8/1257 |
Appears in Collections: | CICECO - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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cells-11-01257 (1).pdf | Endo- and Exometabolome Crosstalk in Mesenchymal Stem Cells Undergoing Osteogenic Differentiation | 4.99 MB | Adobe PDF | View/Open |
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