Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/32769
Title: | Purification of interferon alpha 2b-based biopharmaceuticals using ionic liquid-based technologies |
Author: | Castro, Leonor Lobo, Guilherme Pereira, Patrícia Passarinha, Luís Neves, Márcia C. Freire, Mara G. Pedro, Augusto Q. |
Issue Date: | Nov-2021 |
Publisher: | ISPPP |
Abstract: | Protein biopharmaceuticals, among which interferon alpha-2b (IFNα-2b) that can be used in the treatment of chronic hepatitis C, have become an indispensable product of current medicine. Aiming at finding new cost-effective, efficient and sustainable technologies for recombinant IFNα-2b purification, ionic liquids were investigated as adjuvants in polymer-polymer aqueous two-phase systems (ATPS) or as chromatographic ligands covalently attached in silica (Supported ionic liquids, SILs). The application of ionic liquids as adjuvants in ATPS composed of polyethylene glycol (PEG 600 g/mol) and polypropylene glycol (PPG 400 g/mol) enhanced the purity of IFNα-2b recovered in the PEG-rich phase. On the other hand, SILs exhibited promising results toward IFNα-2b purification both in conditions favouring ionic or hydrophobic interactions, reinforcing the multimodal character of these novel stationary phases. Also, the secondary structure of IFNα-2b is preserved with both purification processes, as appraised by circular dichroism and western-blot studies. Overall, our results demonstrate the high potential exhibited by ionic liquids toward the preparative purification of the recombinant IFNα-2b biopharmaceuticals. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/32769 |
Appears in Collections: | CICECO - Comunicações DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Abstract - ISPPP_IFN.pdf | 121.39 kB | Adobe PDF | View/Open |
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