Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/28550
Title: | Evaluation of in vivo reproductive toxicity of potassium chromate in male mice |
Author: | Oliveira, Helena Spanò, Marcello Guevara, Miguel Angel Santos, Teresa Margarida Santos, Conceição Pereira, Maria de Lourdes |
Keywords: | Potassium chromate Function of sperm cells Acrosome DNA damage of sperm cells Tubular diameter Testicular toxicity Flow cytometry Histopathology |
Issue Date: | Jul-2010 |
Publisher: | Elsevier |
Abstract: | To evaluate the effects of potassium chromate on mice sperm cells after a short-term exposure, male ICR-CD1 mice were administered with 5 or 10mgK(2)CrO(4)/bw for 4 consecutive days. One group of mice was sacrificed at day 5, starting from the beginning of the experiment and another group was sacrificed at day 35. Testis and epididymis histology was evaluated by light microscopy and testicular cells populations were evaluated by flow cytometry (FCM). Spermatozoa were collected from the epididymis and their morphology and several functional parameters (density, motility, viability, mitochondrial function, acrosome integrity) were evaluated. Furthermore, DNA fragmentation and chromatin status of sperm cells were assessed at both experimental periods. Besides a reduction in seminiferous tubules diameter, exposure to potassium chromate did not induce further histopathological changes in mice testis or epididymis. These results were supported by the analysis of testicular cellular subpopulations by FCM. Concerning spermatozoa morphology, an increase in the percentage of multiple abnormalities and a decrease in the percentage of normal spermatozoa were found at days 5 and 35, respectively. Although spermatozoa mitochondrial function or viability was not affected, its motility was significantly reduced by potassium chromate exposure at both experimental periods. A decrease in acrosome integrity was found in mice injected with 10mgK(2)CrO(4)/bw after 35 days. Exposure to potassium chromate did not affect either DNA fragmentation or chromatin susceptibility to acid denaturation of sperm cells. In this work, we were able to show the effects of potassium chromate on spermatozoa physiological parameters such as motility, morphology and acrosome status and also demonstrate that the doses tested did not induce DNA damage to sperm cells after one spermatogenic cycle. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/28550 |
DOI: | 10.1016/j.etp.2009.05.009 |
ISSN: | 0940-2993 |
Appears in Collections: | CESAM - Artigos CICECO - Artigos DBio - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Oliveira et al. - 2010 - Evaluation of in vivo reproductive toxicity of pot.pdf | 830.56 kB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.