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http://hdl.handle.net/10773/26342
Title: | A rapid and inexpensive molecular technique to discriminate the north-eastern Atlantic and Mediterranean Atherina species and its potential applications in ecology and larval identification |
Author: | Carvalho Almada, Vítor Martins Francisco, Sara Rosa, Inês Domingues, Vera Cabral, Henrique Almada, Frederico Henriques, Miguel Robalo, Joana |
Keywords: | Atherina RFLP mtDNA |
Issue Date: | 2012 |
Publisher: | Springer |
Abstract: | This note describes a rapid and inexpensive Restriction Fragment Length Polymorphism technique to discriminate all species of Atherina (Pisces: Atherinidae) of the north-eastern Atlantic and the Mediterranean. This technique is based on digestion of a fragment of the 12S ribosomal RNA (12SrRNA) gene region of mitochondrial DNA with restriction enzymes that recognize species-specific nucleotide sites. The three currently recognized species in the area, as well as two additional forms awaiting formal description, can be discriminated using a set of four endonucleases. We argue that this simple and fast technique may be of great help in the identification of young stages and in ecological surveys. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/26342 |
DOI: | 10.2478/s11756-012-0087-9 |
ISSN: | 0006-3088 |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Almada et al. - 2012 - A rapid and inexpensive molecular technique to dis.pdf | 176.14 kB | Adobe PDF |
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