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http://hdl.handle.net/10773/26342
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DC Field | Value | Language |
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dc.contributor.author | Carvalho Almada, Vítor | pt_PT |
dc.contributor.author | Martins Francisco, Sara | pt_PT |
dc.contributor.author | Rosa, Inês | pt_PT |
dc.contributor.author | Domingues, Vera | pt_PT |
dc.contributor.author | Cabral, Henrique | pt_PT |
dc.contributor.author | Almada, Frederico | pt_PT |
dc.contributor.author | Henriques, Miguel | pt_PT |
dc.contributor.author | Robalo, Joana | pt_PT |
dc.date.accessioned | 2019-07-31T15:29:51Z | - |
dc.date.available | 2019-07-31T15:29:51Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0006-3088 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/26342 | - |
dc.description.abstract | This note describes a rapid and inexpensive Restriction Fragment Length Polymorphism technique to discriminate all species of Atherina (Pisces: Atherinidae) of the north-eastern Atlantic and the Mediterranean. This technique is based on digestion of a fragment of the 12S ribosomal RNA (12SrRNA) gene region of mitochondrial DNA with restriction enzymes that recognize species-specific nucleotide sites. The three currently recognized species in the area, as well as two additional forms awaiting formal description, can be discriminated using a set of four endonucleases. We argue that this simple and fast technique may be of great help in the identification of young stages and in ecological surveys. | pt_PT |
dc.description.sponsorship | The authors are grateful to Dr. B. Castro and Prof. F.J. Mendes Gon¸calves. The authors also thank P. Carpi and F. Carlos for their help in the laboratory. This study was funded by the European Science Foundation’s MarinERA project “Marine phylogeographic structuring during climate change: the signature of leading and rear edge of range shifting populations” and by the Eco-Ethology Research Unit (331/94) pluriannual financing programme (Fundação para a Ciência e Tecnologia, partially FEDER funded). | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Springer | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F12653%2F2003/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63170%2F2009/PT | pt_PT |
dc.rights | restrictedAccess | pt_PT |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.subject | Atherina | pt_PT |
dc.subject | RFLP | pt_PT |
dc.subject | mtDNA | pt_PT |
dc.title | A rapid and inexpensive molecular technique to discriminate the north-eastern Atlantic and Mediterranean Atherina species and its potential applications in ecology and larval identification | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.firstPage | 988 | pt_PT |
degois.publication.issue | 5 | pt_PT |
degois.publication.lastPage | 991 | pt_PT |
degois.publication.title | Biologia | pt_PT |
degois.publication.volume | 67 | pt_PT |
dc.identifier.doi | 10.2478/s11756-012-0087-9 | pt_PT |
dc.identifier.essn | 1336-9563 | pt_PT |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Almada et al. - 2012 - A rapid and inexpensive molecular technique to dis.pdf | 176.14 kB | Adobe PDF |
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