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http://hdl.handle.net/10773/25419
Title: | Heterologous expression, biosynthesis, and mutagenesis of type II lantibiotics from Bacillus licheniformis in Escherichia coli |
Author: | Caetano, Tânia Krawczyk, Joanna M. Mösker, Eva Süssmuth, Roderich D. Mendo, Sónia |
Issue Date: | 2011 |
Publisher: | Elsevier |
Abstract: | Lichenicidin is a class II two-component lantibiotic produced by Bacillus licheniformis. It is composed of the two peptides Bliα and Bliβ, which act synergistically against various Gram-positive bacteria. The lichenicidin gene cluster was successfully expressed in Escherichia coli, thus constituting the first report to our knowledge of a full reconstitution of a lantibiotic biosynthetic pathway in vivo by a Gram-negative host. This system was further exploited to characterize and assign the function of proteins encoded in the biosynthetic gene cluster in the maturation of lichenicidin peptides. Moreover, a trans complementation system was developed for expression of Bliα and Bliβ variants in vivo. This contribution will spur future studies in the heterologous expression and engineering of lantibiotics. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/25419 |
DOI: | 10.1016/j.chembiol.2010.11.010 |
ISSN: | 1074-5521 |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Caetano et al. - 2011 - Heterologous Expression, Biosynthesis, and Mutagen.pdf | 931.3 kB | Adobe PDF |
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