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http://hdl.handle.net/10773/25419
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DC Field | Value | Language |
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dc.contributor.author | Caetano, Tânia | pt_PT |
dc.contributor.author | Krawczyk, Joanna M. | pt_PT |
dc.contributor.author | Mösker, Eva | pt_PT |
dc.contributor.author | Süssmuth, Roderich D. | pt_PT |
dc.contributor.author | Mendo, Sónia | pt_PT |
dc.date.accessioned | 2019-02-22T15:41:58Z | - |
dc.date.available | 2019-02-22T15:41:58Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1074-5521 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/25419 | - |
dc.description.abstract | Lichenicidin is a class II two-component lantibiotic produced by Bacillus licheniformis. It is composed of the two peptides Bliα and Bliβ, which act synergistically against various Gram-positive bacteria. The lichenicidin gene cluster was successfully expressed in Escherichia coli, thus constituting the first report to our knowledge of a full reconstitution of a lantibiotic biosynthetic pathway in vivo by a Gram-negative host. This system was further exploited to characterize and assign the function of proteins encoded in the biosynthetic gene cluster in the maturation of lichenicidin peptides. Moreover, a trans complementation system was developed for expression of Bliα and Bliβ variants in vivo. This contribution will spur future studies in the heterologous expression and engineering of lantibiotics. | pt_PT |
dc.description.sponsorship | T.C. was supported by Fundação para a Ciência e Tecnologia and Medinfar Pharmaceuticals SA grant (SFRH/BDE/15559/2005). The work was supported by Centre for Environmental and Marine Studies (CESAM) of University of Aveiro and the Cluster of Excellence “Unifying concepts in catalysis” granted by the DFG, coordinated by TU Berlin. The authors acknowledge Dr. José C. Duarte for supplying B. licheniformis I89 strain, and Professor Dr. Klaus Hantke from University of Tübingen for supplying E. coli BW25113 and BW25113ΔtolC:kan strains. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBDE%2F15559%2F2005/PT | pt_PT |
dc.rights | restrictedAccess | pt_PT |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.title | Heterologous expression, biosynthesis, and mutagenesis of type II lantibiotics from Bacillus licheniformis in Escherichia coli | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.firstPage | 90 | pt_PT |
degois.publication.issue | 1 | pt_PT |
degois.publication.lastPage | 100 | pt_PT |
degois.publication.title | Cell Chemistry Biology | pt_PT |
degois.publication.volume | 18 | pt_PT |
dc.identifier.doi | 10.1016/j.chembiol.2010.11.010 | pt_PT |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Caetano et al. - 2011 - Heterologous Expression, Biosynthesis, and Mutagen.pdf | 931.3 kB | Adobe PDF |
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