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http://hdl.handle.net/10773/24274
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DC Field | Value | Language |
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dc.contributor.author | Azevedo, Juliana S. N. | pt_PT |
dc.contributor.author | Correia, António | pt_PT |
dc.contributor.author | Henriques, Isabel | pt_PT |
dc.date.accessioned | 2018-10-11T13:57:10Z | - |
dc.date.available | 2018-10-11T13:57:10Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0168-6496 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/24274 | - |
dc.description.abstract | Many members of the genus Psychrobacter are endemic in extremely cold and saline environments and the genus has been described as only marginally successful in warmer habitats. In a previous study the Psychrobacter genus was, unexpectedly, the most frequently isolated bacterial genus from the sea-surface microlayer (SML) and the underlying water (UW) of a temperate estuary (Ria de Aveiro, Portugal). Here we analysed the diversity in Psychrobacter populations inhabiting this estuary. Samples were collected at three dates and three locations from sea-SML and UW. Isolated Psychrobacter strains were welladapted to temperatures and salt concentrations above the ones described as optimal for most members of this genus. Hydrocarbon-degrading potential was not confirmed for these strains. We developed and optimized a reliable and specific denaturing gradient gel electrophoresis (DGGE)-based method for the analysis of Psychrobacter populations in aquatic systems. DGGE profiles inferred that Psychrobacter populations were very stable in the estuary, a strong indication for the presence of well-adapted phylotypes. The analysis of genusspecific clone libraries revealed a surprisingly high diversity among Psychrobacter in Ria de Aveiro. Results indicated that novel species were probably cultivated. Significant differences between sea-SML and UW Psychrobacter communities were revealed. Observed diversity trends may be related to environmental factors such as salinity and/or anthropogenic pressures such as contamination with hydrocarbons. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Blackwell Publishing | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F21384%2F2005/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64057%2F2009/PT | pt_PT |
dc.rights | restrictedAccess | pt_PT |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.subject | 16S rRNA gene libraries | pt_PT |
dc.subject | Denaturing gradient gel electrophoresis | pt_PT |
dc.subject | Estuary | pt_PT |
dc.subject | Psychrobacter | pt_PT |
dc.subject | Psychrophilic | pt_PT |
dc.title | Molecular analysis of the diversity of genus Psychrobacter present within a temperate estuary | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.firstPage | 451 | pt_PT |
degois.publication.issue | 3 | pt_PT |
degois.publication.lastPage | 460 | pt_PT |
degois.publication.title | FEMS Microbiology Ecology | pt_PT |
degois.publication.volume | 84 | pt_PT |
dc.identifier.doi | 10.1111/1574-6941.12075 | pt_PT |
dc.identifier.essn | 1574-6941 | pt_PT |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Azevedo et al. - 2013 - Molecular analysis of the diversity of genus Psych.pdf | 369.62 kB | Adobe PDF |
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