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Título: | Molecular analysis of the diversity of genus Psychrobacter present within a temperate estuary |
Autor: | Azevedo, Juliana S. N. Correia, António Henriques, Isabel |
Palavras-chave: | 16S rRNA gene libraries Denaturing gradient gel electrophoresis Estuary Psychrobacter Psychrophilic |
Data: | 2013 |
Editora: | Blackwell Publishing |
Resumo: | Many members of the genus Psychrobacter are endemic in extremely cold and saline environments and the genus has been described as only marginally successful in warmer habitats. In a previous study the Psychrobacter genus was, unexpectedly, the most frequently isolated bacterial genus from the sea-surface microlayer (SML) and the underlying water (UW) of a temperate estuary (Ria de Aveiro, Portugal). Here we analysed the diversity in Psychrobacter populations inhabiting this estuary. Samples were collected at three dates and three locations from sea-SML and UW. Isolated Psychrobacter strains were welladapted to temperatures and salt concentrations above the ones described as optimal for most members of this genus. Hydrocarbon-degrading potential was not confirmed for these strains. We developed and optimized a reliable and specific denaturing gradient gel electrophoresis (DGGE)-based method for the analysis of Psychrobacter populations in aquatic systems. DGGE profiles inferred that Psychrobacter populations were very stable in the estuary, a strong indication for the presence of well-adapted phylotypes. The analysis of genusspecific clone libraries revealed a surprisingly high diversity among Psychrobacter in Ria de Aveiro. Results indicated that novel species were probably cultivated. Significant differences between sea-SML and UW Psychrobacter communities were revealed. Observed diversity trends may be related to environmental factors such as salinity and/or anthropogenic pressures such as contamination with hydrocarbons. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/24274 |
DOI: | 10.1111/1574-6941.12075 |
ISSN: | 0168-6496 |
Aparece nas coleções: | CESAM - Artigos DBio - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Azevedo et al. - 2013 - Molecular analysis of the diversity of genus Psych.pdf | 369.62 kB | Adobe PDF |
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