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Title: New molecular variants of epsilon and beta IncP-1 plasmids are present in estuarine waters
Author: Oliveira, C. S.
Lázaro, B.
Azevedo, J. S. N.
Henriques, I.
Almeida, A.
Correia, A.
Keywords: Estuary
IncP-1 plasmid
trfA gene
Surface sea microlayer
Ria de Aveiro
Issue Date: 2012
Publisher: Elsevier
Abstract: In this work the presence of broad-host-plasmids in an estuary in Portugal has been inves- tigated. Pseudomonas putida KT2442 was used as model recipient bacteria in biparental matings with tetracycline and mercury to select for resistance phenotypes. As a result, 7 transconjugants were shown to carry broad-host-plasmids from the IncP-1 group, as seen by PCR amplification of the trfA gene. Sequence analysis confirmed the isolation of 4 plas- mids from b -1 subgroup and 3 assigned to the recently described e subgroup. To our knowledge this is the first report concerning the detection and isolation of IncP-1 b and e plasmids in estuarine waters. Moreover it is shown that, even though the retrieved plas- mids are phylogenetically close to previously characterized plasmids, such as pB10 and pKJK5, respectively, they constitute new molecular variants.
Peer review: yes
DOI: 10.1016/j.plasmid.2011.11.002
ISSN: 0147-619X
Appears in Collections:CESAM - Artigos
DBio - Artigos
PT Mar - Artigos
Ria de Aveiro - Artigos

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