Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/11102
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dc.contributor.authorOliveira, C. S.pt
dc.contributor.authorLázaro, B.pt
dc.contributor.authorAzevedo, J. S. N.pt
dc.contributor.authorHenriques, I.pt
dc.contributor.authorAlmeida, A.pt
dc.contributor.authorCorreia, A.pt
dc.date.accessioned2013-10-14T09:41:08Z-
dc.date.issued2012-
dc.identifier.issn0147-619Xpt
dc.identifier.urihttp://hdl.handle.net/10773/11102-
dc.description.abstractIn this work the presence of broad-host-plasmids in an estuary in Portugal has been inves- tigated. Pseudomonas putida KT2442 was used as model recipient bacteria in biparental matings with tetracycline and mercury to select for resistance phenotypes. As a result, 7 transconjugants were shown to carry broad-host-plasmids from the IncP-1 group, as seen by PCR amplification of the trfA gene. Sequence analysis confirmed the isolation of 4 plas- mids from b -1 subgroup and 3 assigned to the recently described e subgroup. To our knowledge this is the first report concerning the detection and isolation of IncP-1 b and e plasmids in estuarine waters. Moreover it is shown that, even though the retrieved plas- mids are phylogenetically close to previously characterized plasmids, such as pB10 and pKJK5, respectively, they constitute new molecular variants.pt
dc.language.isoengpt
dc.publisherElsevierpt
dc.relationFundo Social Europeu - SFRH/BPD/65820/2009pt
dc.relationFundo Social Europeu - SFRH/BPD/21384/2005pt
dc.relationFundo Social Europeu - FRH/BD/64057/2009pt
dc.relationMinistério da Ciência, Tecnologia e Ensino Superior, Portugalpt
dc.rightsrestrictedAccesspor
dc.subjectEstuarypt
dc.subjectIncP-1 plasmidpt
dc.subjectBroad-host-range-plasmidpt
dc.subjecttrfA genept
dc.subjectSurface sea microlayerpt
dc.subjectRia de Aveiropt
dc.titleNew molecular variants of epsilon and beta IncP-1 plasmids are present in estuarine waterspt
dc.typearticlept
dc.peerreviewedyespt
ua.distributioninternationalpt
ua.event.titlePlasmid-
degois.publication.firstPage252pt
degois.publication.issue3pt
degois.publication.lastPage258pt
degois.publication.titlePlasmidpt
degois.publication.volume67pt
dc.date.embargo10000-01-01-
dc.identifier.doi10.1016/j.plasmid.2011.11.002pt
Appears in Collections:CESAM - Artigos
DBio - Artigos
PT Mar - Artigos
Ria de Aveiro - Artigos

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