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http://hdl.handle.net/10773/7735
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DC Field | Value | Language |
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dc.contributor.author | Saraiva, Jorge | pt |
dc.contributor.author | Oliveira, Jorge C. | pt |
dc.contributor.author | Oliveira, Susana | pt |
dc.contributor.author | Hendrickx, Marc | pt |
dc.date.accessioned | 2012-04-04T11:14:13Z | - |
dc.date.issued | 1996 | - |
dc.identifier.issn | 1365-2621 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/7735 | - |
dc.description.abstract | The thermal stability of horseradish peroxidase suspensions was studied in three organic solvents of different hydrophobicity (dodecane, octane, and 1-octanol) at three different water contents (14.1, 55.3 and 256.2mg water g−1 dry protein). In these conditions, the enzyme is much more stable than in aqueous solutions (inactivation temperatures were in the range of 125–150°C). The enzyme showed a similar stability when in the presence of organic solvents, compared to the enzyme in a solid matrix without organic solvents with the same water content. The inactivation kinetics was well described by assuming the existence of two iso-enzymes, both inactivating according to a First order model. The lowest value for the z-value of both fractions (around 15°C) was obtained at the higher water content studied. The use of solvent and water content variables should be adequate to develop time-temperature integrators to monitor thermal processes at 100–140°C. | pt |
dc.language.iso | eng | pt |
dc.publisher | Wiley-Blackwell | pt |
dc.relation | AAIR programme - AIR1-CT92-0746 | pt |
dc.relation | JNICT | pt |
dc.rights | restrictedAccess | por |
dc.subject | Biocatalysis | pt |
dc.subject | Enzyme thermal inactivation | pt |
dc.subject | Time-temperature integrators | pt |
dc.subject | Water activity | pt |
dc.title | Inactivation kinetics of horseradish peroxidase in organic solvents of different hydrophobicity at different water contents | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 233 | pt |
degois.publication.issue | 3 | |
degois.publication.issue | 3 | pt |
degois.publication.lastPage | 240 | pt |
degois.publication.title | International Journal of Food Science and Technology | pt |
degois.publication.volume | 31 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.doi | 10.1046/j.1365-2621.1996.00343.x | pt |
dc.identifier.doi | 10.1046/j.1365-2621.1996.00343.x | pt |
Appears in Collections: | QOPNA - Artigos |
Files in This Item:
File | Description | Size | Format | |
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P2_IJFST_Peroxidase organic solvents.pdf | 600.58 kB | Adobe PDF |
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