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http://hdl.handle.net/10773/7036
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DC Field | Value | Language |
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dc.contributor.author | Machado, Maria Fátima | pt |
dc.contributor.author | Saraiva, Jorge Manuel | pt |
dc.date.accessioned | 2012-02-28T18:28:16Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0141-5492 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/7036 | - |
dc.description.abstract | Thermal deactivation kinetics of horseradish peroxidase (HRP) were studied from 45 to 90 degrees C in phosphate buffer and 5-25% (v,w/v) 1-butyl-3-methylimidazolium tetrafluoroborate [BMIM][BF4] and 1-butyl-3-methylimidazolium chloride [BMIM][Cl]. HRP activity at 25 degrees C was not affected by the presence of ionic liquids up to 20% (v,w/v). Increasing the ionic liquids concentration up to 25% (v,w/v) changed the biphasic character of deactivation kinetics to an apparent single first-order step. The presence of 5-10% (v/v) [BMIM][BF4] significantly improved HRP thermal stability with lower activation energies for the deactivation second phase (83-87 kJ mol(-1)). After deactivation, enhanced activity regain of the enzyme, up to 70-80% of the initial activity, was found in 25% (v/v) [BMIM][BF4] and 10% (w/v) [BMIM][Cl] and correlated to prevalence of the deactivation first phase. | pt |
dc.language.iso | eng | pt |
dc.publisher | Springer Verlag | pt |
dc.relation | FCT - SFRH/BPD/11458/2002 | pt |
dc.rights | restrictedAccess | por |
dc.subject | Activity regain | pt |
dc.subject | Horseradish peroxidase | pt |
dc.subject | Ionic liquids | pt |
dc.subject | Kinetic parameters | pt |
dc.subject | Thermal deactivation | pt |
dc.title | Thermal stability and activity regain of horseradish peroxidase in aqueous mixtures of imidazolium-based ionic liquids | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 1233 | pt |
degois.publication.issue | 16 | - |
degois.publication.issue | 16 | pt |
degois.publication.lastPage | 1239 | pt |
degois.publication.title | Biotechnology Letters | pt |
degois.publication.volume | 27 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.doi | 10.1007/s10529-005-0023-y | * |
Appears in Collections: | QOPNA - Artigos |
Files in This Item:
File | Description | Size | Format | |
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P13_BL_ Peroxidase IL.pdf | 263.87 kB | Adobe PDF |
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