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http://hdl.handle.net/10773/5513
Title: | Ras gene in marine mussels: A molecular level response to petrochemical exposure |
Author: | Lima, I. Peck, M. R. Rendon-Von Osten, J. Soares, A. M. V. M. Guilhermino, L. Rotchell, J. M. |
Keywords: | Ras gene Mytilus galloprovincialis Mutation Biomarker Genotoxicity Petroleum hydrocarbon |
Issue Date: | 2008 |
Publisher: | Elsevier |
Abstract: | Mussels are susceptible to numerous toxicants and are often employed as bioindicators. This study investigated the status of the ras proto-oncogene in Mytilus galloprovincialis following petrochemical exposure. A M. galloprovincialis homologue of the vertebrate ras gene was isolated, showing conserved sequence in regions of functional importance and a high incidence of polymorphic variation. Mutational damage was investigated in mussels chronically exposed to the water-accommodated fraction of #4 fuel-oil (WAF), and in mussels collected along the NW coast of Portugal in sites with different levels of petrochemical contamination. A ras gene point mutation was identified in the codon 35 of one individual exposed to 12.5% WAF No mutations were detected in mussels from the WAF control or environmental samples. This represents the first report of a ras gene mutation, experimentally-induced by petrochemical exposure, in an invertebrate species. (c) 2008 Elsevier Ltd. All rights reserved. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/5513 |
DOI: | 10.1016/j.marpolbul.2008.01.018 |
ISSN: | 0025-326X |
Appears in Collections: | DBio - Artigos PT Mar - Artigos |
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148_MPB_2008_56_633.pdf | 645.55 kB | Adobe PDF | ![]() |
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