Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/41908
Title: | Bioactive self-regulated liquified microcompartments to bioengineer bone-like microtissues |
Author: | Pinho, Ana R. Gomes, Maria C. Costa, Dora C. S. Mano, João F. |
Keywords: | Catechol analogs Gelatin Mineralization Osteoclastogenesis Osteogenesis |
Issue Date: | 1-Mar-2024 |
Publisher: | Wiley |
Abstract: | Designing a microenvironment that drives autonomous stromal cell differentiation toward osteogenesis while recapitulating the complexity of bone tissue remains challenging. In the current study, bone-like microtissues are created using electrohydrodynamic atomization to form two distinct liquefied microcapsules (mCAPs): i) hydroxypyridinone (HOPO)-modified gelatin (GH mCAPs, 7.5% w/v), and ii) HOPO-modified gelatin and dopamine-modified gelatin (GH+GD mCAPs, 7.5%+1.5% w/v). The ability of HOPO to coordinate with iron ions at physiological pH allows the formation of a semipermeable micro-hydrogel shell. In turn, the dopamine affinity for calcium ions sets a bioactive milieu for bone-like microtissues. After 21 days post encapsulation, GH and GH+GD mCAPs potentiate autonomous osteogenic differentiation of mesenchymal stem cells accompanied by collagen type-I gene upregulation, increased alkaline phosphatase (ALP) expression, and formation of mineralized extracellular matrix. However, the GH+GD mCAPs show higher levels of osteogenic markers starting on day 14, translating into a more advanced and organized mineralized matrix. The GH+GD system also shows upregulation of the receptor activator of nuclear factor kappa-B ligand (RANK-L) gene, enabling the autonomous osteoclastic differentiation of monocytes. These catechol-based mCAPs offer a promising approach to designing multifunctional and autonomous bone-like microtissues to study in vitro bone-related processes at the cell-tissue interface, angiogenesis, and osteoclastogenesis. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/41908 |
DOI: | 10.1002/smll.202305029 |
ISSN: | 1613-6810 |
Appears in Collections: | CICECO - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Bioactive self-regulated liquified microcompartments to bioengineer bone-like microtissues.pdf | 3.19 MB | Adobe PDF | ![]() |
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