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|An Overview on the Recent Advances in Alternative Solvents as Stabilizers of Proteins and Enzymes
|Almeida, Jéssica S.
Capela, Emanuel V.
Loureiro, Ana M.
Tavares, Ana P. M.
Freire, Mara G.
|Currently, the use of alternative solvents is increasing, namely ionic liquids (ILs) and deep eutectic solvents (DESs) in diverse fields of knowledge, such as biochemistry, chemistry, chemical engineering, biotechnology and biomedicine. Particularly, when compared to traditional solvents, these alternative solvents have great importance for biomolecules due to the enhanced solubility, structure stability and the biological activity of biomolecules, such as protein and enzymes. Thus, in this review article, the recent developments and efforts on the technological developments carried out with ILs and DESs for the stabilization and activation of proteins and enzymes are provided. The most studied IL-and DES-based formulations for proteins and enzymes are discussed and the molecular mechanisms and interactions related to the increased stability promoted by these alternative solvents are disclosed, while emphasizing their main advantages
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|CICECO - Artigos
DQ - Artigos
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