Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/32877
Title: | Purification of interferon alfa-2b using ionic liquid-based technologies |
Author: | Castro, Leonor S. Sousa, Guilherme L. Pereira, Patrícia Neves, Márcia C. Freire, Mara G. Pedro, Augusto Q. |
Keywords: | Biopharmaceuticals Purification Supported ionic liquids Chromatography Aqueous twophase systems IFNα-2b |
Issue Date: | Jun-2021 |
Publisher: | FEUP |
Abstract: | The development of protein-based biopharmaceuticals and their introduction on modern medicine brought enormous benefits to the treatment of life-threatening diseases such as cancer, diabetes and neurodegenerative disorders. However, their current high-cost still limits their widespread use. While significant advances at the upstream level have occurred, current purification strategies are unable to cope with high product concentrations resorting to cost-effective approaches. This work entails the development of sustainable downstream processing methodologies for recombinant interferon (IFN) α-2b derived from Escherichia coli using ionic liquids, either by exploring their application as adjuvants in polymer-polymer aqueous two-phase systems (ATPS) or as chromatographic ligands covalently attached in silica (supported ionic liquids - SILs). Overall, our results demonstrate the high potential exhibited by ionic liquids toward the preparative purification of the recombinant IFN α-2b biopharmaceutical. |
Peer review: | no |
URI: | http://hdl.handle.net/10773/32877 |
Appears in Collections: | CICECO - Comunicações DQ - Comunicações |
Files in This Item:
File | Description | Size | Format | |
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DCE21_Abstract.pdf | 180.12 kB | Adobe PDF | View/Open |
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