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http://hdl.handle.net/10773/30408
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DC Field | Value | Language |
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dc.contributor.author | Dias, Reyla A. S. | pt_PT |
dc.contributor.author | Sousa, Eliane R. | pt_PT |
dc.contributor.author | Silva, Gilmar S. | pt_PT |
dc.contributor.author | Silva, Lanna K. | pt_PT |
dc.contributor.author | Freitas, Arlan S. | pt_PT |
dc.contributor.author | Lima, Diana L. D. | pt_PT |
dc.contributor.author | Sousa, Érika M. L. | pt_PT |
dc.date.accessioned | 2021-01-29T13:21:53Z | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 0026-265X | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/30408 | - |
dc.description.abstract | This work describes the development of an HPLC-FLD methodology for the separation of five fluoroquinolones (ciprofloxacin, enrofloxacin, sarafloxacin, norfloxacin and levofloxacin) followed by optimization of the DLLME process for the clean-up and preconcentration of enrofloxacin in samples of seawater and river water. The mobile phase used for the chromatographic separation consisted of methanol: phosphate buffer (NaHPO4 H2O 0.04 M pH 3 with H3PO4 85 %), gradient eluted at a ratio of 20:80 (v:v). The mobile phase flow was maintained at 1.2 mL min-1. For the ultrasonic-assisted dispersive liquid liquid microextraction (UA-DLLME), the following conditions were used: 8 mL of sample with pH adjusted to 8, extraction solvent: 500 μL of chloroform, dispersive solvent: 500 μL of acetonitrile; samples were vortexed and sonicated for 2 minutes, each. The enrichment factor (EF) was 54.7 and the recovery was 70 %, achieving a limit of detection (LOD) of 0.11 μg L-1. Repeatability and intermediate reproducibility presented values of relative standard deviation (RSD) lower than 2 %. Finally, the optimized method was applied to the analysis of water and enrofloxacin was detected in both water samples with a concentration of 0.20 μg L-1 in the river and 0.12 μg L-1 in the seawater. However, recovery tests performed to evaluate the water matrices' effects on the extraction performance, presented recoveries of 72±6.1 for river water and 27±8.3 for seawater. These results demonstrate that hereby developed method is only suitable for water samples with a low salinity content. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | UIDB/50017/2020 | pt_PT |
dc.relation | UIDP/50017/2020 | pt_PT |
dc.relation | PTDC/ASP-PES/29021/2017 | pt_PT |
dc.rights | openAccess | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
dc.subject | Pharmaceuticals | pt_PT |
dc.subject | Fluoroquinolones | pt_PT |
dc.subject | High-performance liquid chromatography | pt_PT |
dc.subject | Water samples | pt_PT |
dc.subject | Dispersive liquid-liquid microextraction | pt_PT |
dc.title | Ultrasound-assisted dispersive liquid-liquid microextraction for determination of enrofloxacin in surface waters | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.issue | Part A | pt_PT |
degois.publication.title | Microchemical Journal | pt_PT |
degois.publication.volume | 160 | pt_PT |
dc.identifier.doi | 10.1016/j.microc.2020.105633 | pt_PT |
Appears in Collections: | CESAM - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Pre-proofs.pdf | 1.06 MB | Adobe PDF | View/Open |
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