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Title: Impact of organic and inorganic nanomaterials in the soil microbial community structure
Author: Nogueira, Verónica
Lopes, Isabel
Rocha-Santos, Teresa
Santos, Ana L.
Rasteiro, Graça M.
Antunes, Filipe
Gonçalves, Fernando
Soares, Amadeu M. V. M.
Cunha, Angela
Almeida, Adelaide
Gomes, Newton N. C. M.
Pereira, Ruth
Keywords: Soil bacterial community
Structural diversity
PCR-DGGE analysis
Issue Date: 1-May-2012
Publisher: Elsevier
Abstract: In this study the effect of organic and inorganic nanomaterials (NMs) on the structural diversity of the soil microbial community was investigated by Denaturing Gradient Gel Electrophoresis, after amplification with universal primers for the bacterial region V6-V8 of 16S rDNA. The polymers of carboxylmethyl-cellulose (CMC), of hydrophobically modified CMC (HM-CMC), and hydrophobically modified polyethylglycol (HM-PEG); the vesicles of sodium dodecyl sulphate/didodecyl dimethylammonium bromide (SDS/DDAB) and of monoolein/sodium oleate (Mo/NaO); titanium oxide (TiO(2)), titanium silicon oxide (TiSiO(4)), CdSe/ZnS quantum dots, gold nanorods, and Fe/Co magnetic fluid were the NMs tested. Soil samples were incubated, for a period of 30 days, after being spiked with NM suspensions previously characterized by Dynamic Light Scattering (DLS) or by an ultrahigh-resolution scanning electron microscope (SEM). The analysis of similarities (ANOSIM) of DGGE profiles showed that gold nanorods, TiO(2), CMC, HM-CMC, HM-PEG, and SDS/DDAB have significantly affected the structural diversity of the soil bacterial community.
Peer review: yes
DOI: 10.1016/j.scitotenv.2012.02.041
ISSN: 0048-9697
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