Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/25503
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dc.contributor.authorSemitela, Ângelapt_PT
dc.contributor.authorGirão, Andrépt_PT
dc.contributor.authorFernandes, Carlapt_PT
dc.contributor.authorCompleto, Antóniopt_PT
dc.contributor.authorMarques, Paulapt_PT
dc.date.accessioned2019-03-07T12:11:39Z-
dc.date.available2019-03-07T12:11:39Z-
dc.date.issued2018-07-
dc.identifier.isbn978-972-789-547-2-
dc.identifier.urihttp://hdl.handle.net/10773/25503-
dc.description.abstractPolycaprolactone (PCL) electrospun scaffolds have long been used for cartilage tissue engineering applications due to their biocompatibility, biodegradability, good mechanical properties and easy processability. However, their inherent hydrophobicity prevents cell adhesion and cell proliferation. On the other hand, natural polymers, such as gelatin, have been reported to support cell adhesion due to its hydrophilic character and the presence of cell recognition sites. Another common limitation of PCL electrospun scaffolds is their inherent small pores, which can hinder cell migration. The introduction of a sacrificial agent on the scaffolds, such as polyethylene glycol (PEG), which can be co-electrospun with the polymer of interest, has been reported to overcome this limitation. The sacrificial polymer is then dissolved away in water, resulting in an electrospun scaffolds with increased porosity. The present work combines these approaches to improve the surface properties and the scaffolds’ porosity that will benefit cell adhesion, migration and proliferation. Thus, a new series of electrospun scaffolds composed of PCL, gelatin and PEG sacrificial particles were fabricated and characterized on their chemical composition, wettability, topography and biocompatibility using an articular cartilage progenitor cell line. According to the results obtained, the addition of gelatin led to an increased hydrophilicity of the scaffolds, which resulted in better cell adhesion and proliferation. The introduction of PEG sacrificial particles enlarged the pore size of the scaffolds to values comparable to the cell diameter and allowed cell migration through the scaffold.pt_PT
dc.language.isoengpt_PT
dc.publisherUA Editorapt_PT
dc.relationPOCI-01-0145- FEDER-016574pt_PT
dc.relationPTDC/EMS-TEC/3263/2014pt_PT
dc.rightsopenAccesspt_PT
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCartilage tissue engineeringpt_PT
dc.subjectElectrospinningpt_PT
dc.subjectPCLpt_PT
dc.subjectGelatinpt_PT
dc.subjectPEGpt_PT
dc.titleImproving surface properties and porosity of electrospun scaffolds for cartilage tissue engineeringpt_PT
dc.typeconferenceObjectpt_PT
dc.description.versionpublishedpt_PT
dc.peerreviewedyespt_PT
ua.event.date16-18 de julho de 2018pt_PT
degois.publication.firstPage72pt_PT
degois.publication.lastPage72pt_PT
degois.publication.locationAveiropt_PT
degois.publication.titleInternational Conference on Nanomaterials Science and Mechanical Engineering Book of Abstractspt_PT
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