Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/24271
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lourenço, Joana | pt_PT |
dc.contributor.author | Pereira, Ruth | pt_PT |
dc.contributor.author | Gonçalves, Fernando | pt_PT |
dc.contributor.author | Mendo, Sónia | pt_PT |
dc.date.accessioned | 2018-10-11T11:36:28Z | - |
dc.date.available | 2018-10-11T11:36:28Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0048-9697 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/24271 | - |
dc.description.abstract | Genotoxic effects caused by the exposure to wastes containing metals and radionuclides were investigated in the European wood mice (Apodemus sylvaticus). The animals were captured in the surroundings of an abandoned uranium mining site. DNA damage was assessed by comet assay; gene expression and single nucleotide polymorphisms (SNPs) were assessed, respectively, by Real-Time PCR and melt curve analysis. The bioaccumulation of metals in the liver, kidney and bones was also determined to help clarify cause-effect relationships. Results confirmed the bioaccumulation of cadmium and uranium in organisms exposed to uranium mining wastes. P53 gene was found to be significantly up-regulated in the liver of those organisms and SNPs in the Rb gene were also detected in the kidney. Our results showed that uranium mining wastes caused serious DNA damage resulting in genomic instability, disclosed by the significant increase in DNA strand breaks and P53 gene expression disturbance. These effects can have severe consequences, since they may contribute for the emergence of serious genetic diseases. The fact that mice are often used as bioindicator species for the evaluation of risks of environmental exposure to humans, raises concerns on the risks for human populations living near uranium mining areas. | pt_PT |
dc.description.sponsorship | Fundação para a Ciência e Tecnologia (Portuguese Ministry of Science, Technology and Higher Education) has funded this study through a PhD Grant to Joana Lourenço (ref. SFRH/BD/37007/2007) and through the research Project ENGENUR (ref. PTDC/AAC-AMB/114057/2009). This research was also funded by FEDER through COMPETE and also partially funded by FSE, POPH funds (Programa Ciência 2007) and the Portuguese Government (Programa 16 Ciência Inovação 2010). | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F37007%2F2007/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/114057/PT | pt_PT |
dc.rights | restrictedAccess | pt_PT |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | pt_PT |
dc.subject | Bioaccumulation | pt_PT |
dc.subject | DNA damage | pt_PT |
dc.subject | Gene expression | pt_PT |
dc.subject | Metals | pt_PT |
dc.subject | Radionuclides | pt_PT |
dc.subject | SNPs | pt_PT |
dc.title | Metal bioaccumulation, genotoxicity and gene expression in the European wood mouse (Apodemus sylvaticus) inhabiting an abandoned uranium mining area | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.firstPage | 673 | pt_PT |
degois.publication.lastPage | 680 | pt_PT |
degois.publication.title | Science of the Total Environment | pt_PT |
degois.publication.volume | 443 | pt_PT |
dc.identifier.doi | 10.1016/j.scitotenv.2012.10.105 | pt_PT |
dc.identifier.essn | 1879-1026 | pt_PT |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Lourenco et al. - 2013 - Metal bioaccumulation, genotoxicity and gene expre.pdf | 732.32 kB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.