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http://hdl.handle.net/10773/23376
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DC Field | Value | Language |
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dc.contributor.author | Silva, Yolanda J. | pt |
dc.contributor.author | Costa, Liliana | pt |
dc.contributor.author | Pereira, Carla | pt |
dc.contributor.author | Cunha, Ângela | pt |
dc.contributor.author | Calado, Ricardo | pt |
dc.contributor.author | Gomes, Newton C. M. | pt |
dc.contributor.author | Almeida, Adelaide | pt |
dc.date.accessioned | 2018-06-05T11:02:48Z | - |
dc.date.available | 2018-06-05T11:02:48Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1751-7915 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/23376 | - |
dc.description.abstract | Aquaculture facilities worldwide continue to experi-ence significant economic losses because of disease caused by pathogenic bacteria, including multidrug-resistant strains. This scenario drives the search for alternative methods to inactivate pathogenic bacteria. Phage therapy is currently considered as a viable alternative to antibiotics for inactivation of bacterial pathogens in aquaculture systems. While phage therapy appears to represent a useful and flexible tool for microbiological decontamination of aquaculture effluents, the effect of physical and chemical proper-ties of culture waters on the efficiency of this tech-nology has never been reported. The present study aimed to evaluate the effect of physical and chemical properties of aquaculture waters (e.g. pH, tempera-ture, salinity and organic matter content) on the efficiency of phage therapy under controlled experi-mental conditions in order to provide a basis for the selection of the most suitable protocol for subse-quent experiments. A bioluminescent genetically transformed Escherichia coli was selected as a model microorganism to monitor real-time phage therapy kinetics through the measurement of bioluminescence, thus avoiding the laborious and time-consuming conventional method of counting colony-forming units (CFU). For all experiments, a bacterial concentration of ≈ 10 5 CFU ml −1 and a phage concentration of ≈ 10 6–8 plaque forming unit ml −1 were used. Phage survival was not significantly affected by the natural variability of pH (6.5–7.4), temperature (10–25°C), salinity (0–30 g NaCl l −1) and organic matter concentration of aquaculture waters in a tem-perate climate. Nonetheless, the efficiency of phage therapy was mostly affected by the variation of salin-ity and organic matter content. As the effectiveness of phage therapy increases with water salt content, this approach appears to be a suitable choice for marine aquaculture systems. The success of phage therapy may also be enhanced in non-marine systems through the addition of salt, whenever this option is feasible and does not affect the survival of aquatic species being cultured. | pt |
dc.language.iso | eng | pt |
dc.publisher | John Wiley & Sons | pt |
dc.relation | FCOMP-01–0124-FEDER-013934 | pt |
dc.relation | PROMAR 31-03-05-FEP-0028 | pt |
dc.relation | Pest-C/MAR/LA0017/2011 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F65147%2F2009/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F76414%2F2011/PT | pt |
dc.rights | openAccess | por |
dc.title | Influence of environmental variables in the efficiency of phage therapy in aquaculture | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 401 | pt |
degois.publication.issue | 5 | |
degois.publication.issue | 5 | pt |
degois.publication.lastPage | 413 | pt |
degois.publication.title | Microbial Biotechnology | pt |
degois.publication.volume | 7 | pt |
dc.identifier.doi | 10.3166/10.1111/1751-7915.12090 | pt |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Silva et al. - 2014 - Influence of environmental variables in the effici.pdf | 457.74 kB | Adobe PDF | View/Open |
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