Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/20768
Title: | Chitin-glucan complex production by Komagataella pastoris: Downstream optimization and product characterization |
Author: | Farinha, Ines Duarte, Paulo Pimentel, Ana Plotnikova, Evgeniya Chagas, Barbara Mafra, Luis Grandfils, Christian Freitas, Filomena Fortunato, Elvira Reis, Maria A. M. |
Keywords: | YEAST-CELL WALL SACCHAROMYCES-CEREVISIAE BREWERS-YEAST CHITOSAN CULTIVATION TEMPERATURE FUNGI FILMS |
Issue Date: | 2015 |
Publisher: | ELSEVIER SCI LTD |
Abstract: | Purified chitin-glucan complex (CGC(pure)) was extracted from Komagataella pastoris biomass using a hot alkaline treatment, followed by neutralization and repeated washing with deionized water. The copolymer thus obtained had a beta-glucan:chitin molar ratio of 75:25 and low protein and inorganic salts contents (3.0 and 0.9 wt%, respectively). CGC(pure) had an average molecular weight of 4.9 x 10(5) Da with a polydispersity index of 1.7, and a crystallinity index of 50%. Solid-state NMR provided structural insight at the co-polymer. X-ray diffraction suggests that CGC(pure) has alpha-chitin in its structure. CGC(pure) presented an endothermic decomposition peak at 315 degrees C, assigned to the degradation of the saccharide structures. This study revealed that K. pastoris CGC has properties similar to other chitinous biopolymers and may represent an attractive alternative to crustacean chitin derived-products, being a reliable raw material for the development of new/improved pharmaceutical, cosmetic or food products. (C) 2015 Elsevier Ltd. All rights reserved. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/20768 |
DOI: | 10.1016/j.carbpol.2015.05.034 |
ISSN: | 0144-8617 |
Publisher Version: | 10.1016/j.carbpol.2015.05.034 |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Chitin-glucan complex production by Komagataella pastoris Downstream optimization and product characterization_10.1016j.carbpol.2015.05.034.pdf | 1.13 MB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.