Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/20639
Title: | Aqueous two-phase systems based on cholinium salts and tetrahydrofuran and their use for lipase purification |
Author: | Souza, Ranyere L. Lima, Rafaella A. Coutinho, Joao A. P. Soares, Cleide M. F. Lima, Alvaro S. |
Keywords: | IONIC LIQUIDS BIPHASIC SYSTEMS POLYETHYLENE-GLYCOL PROTEIN SEPARATION ASPERGILLUS-NIGER MICROBIAL LIPASES EXTRACTION ECOTOXICITY BIODEGRADATION ACETONITRILE |
Issue Date: | 2015 |
Publisher: | ELSEVIER SCIENCE BV |
Abstract: | Aqueous two-phase systems (ATPS) formed with cholinium-based ionic liquid - ILs (or salts) are a novel, low cost, and high efficient technique for the recovery of biomolecules. This study examines the formation of ATPS based on cholinium-based salts (cholinium chloride, cholinium bitartrate and cholinium dihydrogencitrate) and tetrahydrofuran (THF) for the purification of lipase from Bacillus sp. ITP-001, produced by submerged fermentation. The optimum conditions for this purification were determined to be 40 wt% of THF and 30 wt% of cholinium bitartrate at 25 degrees C. A purification factor of 130.1 +/- 11.7 fold, a lipase yield of 90.0 +/- 0.7% and a partition coefficient of enzyme for IL-rich phase (K-E = 0.11 +/- 0.01) and protein contaminants for THF-rich phase (K-P = 1.16 +/- 0.1) were achieved. (C) 2015 Elsevier B.V. All rights reserved. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/20639 |
DOI: | 10.1016/j.seppur.2015.05.021 |
ISSN: | 1383-5866 |
Publisher Version: | 10.1016/j.seppur.2015.05.021 |
Appears in Collections: | CICECO - Artigos |
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File | Description | Size | Format | |
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Aqueous two-phase systems based on cholinium salts and tetrahydrofuran and their use for lipase purification_10.1016j.seppur.2015.05.021.pdf | 1.19 MB | Adobe PDF |
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