Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/20440
Title: | Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed |
Author: | Queiroga, A. Catarina Pintado, Manuela E. Malcata, F. Xavier |
Keywords: | SHEEP FLEECE ALKALINE PROTEASES AGAR PLATES STRATEGIES ENZYMES ROT |
Issue Date: | 2013 |
Publisher: | ELSEVIER SCIENCE BV |
Abstract: | The main purpose of this study was to isolate and briefly characterize proteolytic bacteria from a poorly known habitat - raw wool. Fleece samples were accordingly collected from Merino raw wool - a Portuguese ewe breed, at three distinct areas of their body, from animals exhibiting no symptoms or signs of abnormalities: they were then subjected to enumeration and isolation of a total of 158 bacterial strains. Said isolates were screened for protease activity, using the spot technique, on Calcium Caseinate Agar containing 1% (w/v) skim milk. The 36 isolates displaying the highest protease activity underwent a more refined assessment of enzymatic performance - by examining their cell-free supernatant extracts, using casein as substrate. Two Bacillus isolates were eventually chosen owing to their highest proteolytic activities (24.6 and 15.9 U/mL), and identified using molecular biology tools. (C) 2012 Elsevier B.V. All rights reserved. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/20440 |
DOI: | 10.1016/j.smallrumres.2012.09.005 |
ISSN: | 0921-4488 |
Publisher Version: | 10.1016/j.smallrumres.2012.09.005 |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Wool-associated proteolytic bacteria isolated from Portuguese Merino breed_10.1016j.smallrumres.2012.09.005.pdf | 571.36 kB | Adobe PDF |
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