Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/19987
Title: | Improving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems |
Author: | Ferreira, Ana M. Faustino, Vania F. M. Mondal, Dibyendu Coutinho, Joao A. P. Freire, Mara G. |
Keywords: | 2-PHASE SYSTEMS MONOCLONAL-ANTIBODIES PROTEINS OPTIMIZATION SEPARATION STABILITY RECOVERY IGG |
Issue Date: | 2016 |
Publisher: | ELSEVIER SCIENCE BV |
Abstract: | Immunoglobulins G (IgG) could become widespread biopharmaceuticals if cost-efficient processes for their extraction and purification are available. In this work, aqueous biphasic systems (ABS) composed of polyethylene glycols and a buffered salt, and with ionic liquids (ILs) as adjuvants, have been studied as alternative extraction and purification platforms of IgG from a rabbit serum source. Eleven ILs were investigated to provide insights on the chemical features which maximize the IgG partitioning. It is shown that in polymer-salt systems pure IgG preferentially partitions to the polymer-rich phase; yet, the complete extraction was never attained. Remarkably, after the addition of 5 wt% of adequate ILs to polymer-salt ABS, the complete extraction of pure IgG in a single-step was accomplished. The best systems and conditions were then applied to the extraction and purification of IgG directly from rabbit serum samples. The complete extraction of IgG in a single-step was maintained while its purity in the polymer-rich phase was enhanced by ca. 37% as compared to the IL-free ABS. The antibody stability was also evaluated revealing that appropriate ILs are able to maintain the IgG stability and can be used as phase-forming components of ABS when envisaging the purification of high-cost biopharmaceuticals. (C) 2016 Elsevier B.V. All rights reserved. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/19987 |
DOI: | 10.1016/j.jbiotec.2016.08.015 |
ISSN: | 0168-1656 |
Publisher Version: | 10.1016/j.jbiotec.2016.08.015 |
Appears in Collections: | CICECO - Artigos |
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File | Description | Size | Format | |
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Improving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems_10.1016j.jbiotec.2016.08.015.pdf | 1.15 MB | Adobe PDF |
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