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http://hdl.handle.net/10773/19987
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DC Field | Value | Language |
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dc.contributor.author | Ferreira, Ana M. | pt |
dc.contributor.author | Faustino, Vania F. M. | pt |
dc.contributor.author | Mondal, Dibyendu | pt |
dc.contributor.author | Coutinho, Joao A. P. | pt |
dc.contributor.author | Freire, Mara G. | pt |
dc.date.accessioned | 2017-12-07T19:31:37Z | - |
dc.date.issued | 2016 | pt |
dc.identifier.issn | 0168-1656 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/19987 | - |
dc.description.abstract | Immunoglobulins G (IgG) could become widespread biopharmaceuticals if cost-efficient processes for their extraction and purification are available. In this work, aqueous biphasic systems (ABS) composed of polyethylene glycols and a buffered salt, and with ionic liquids (ILs) as adjuvants, have been studied as alternative extraction and purification platforms of IgG from a rabbit serum source. Eleven ILs were investigated to provide insights on the chemical features which maximize the IgG partitioning. It is shown that in polymer-salt systems pure IgG preferentially partitions to the polymer-rich phase; yet, the complete extraction was never attained. Remarkably, after the addition of 5 wt% of adequate ILs to polymer-salt ABS, the complete extraction of pure IgG in a single-step was accomplished. The best systems and conditions were then applied to the extraction and purification of IgG directly from rabbit serum samples. The complete extraction of IgG in a single-step was maintained while its purity in the polymer-rich phase was enhanced by ca. 37% as compared to the IL-free ABS. The antibody stability was also evaluated revealing that appropriate ILs are able to maintain the IgG stability and can be used as phase-forming components of ABS when envisaging the purification of high-cost biopharmaceuticals. (C) 2016 Elsevier B.V. All rights reserved. | pt |
dc.language.iso | eng | pt |
dc.publisher | ELSEVIER SCIENCE BV | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147332/PT | pt |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/337753/EU | pt |
dc.rights | restrictedAccess | por |
dc.subject | 2-PHASE SYSTEMS | pt |
dc.subject | MONOCLONAL-ANTIBODIES | pt |
dc.subject | PROTEINS | pt |
dc.subject | OPTIMIZATION | pt |
dc.subject | SEPARATION | pt |
dc.subject | STABILITY | pt |
dc.subject | RECOVERY | pt |
dc.subject | IGG | pt |
dc.title | Improving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 166 | pt |
degois.publication.lastPage | 175 | pt |
degois.publication.title | JOURNAL OF BIOTECHNOLOGY | pt |
degois.publication.volume | 236 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.relation.publisherversion | 10.1016/j.jbiotec.2016.08.015 | pt |
dc.identifier.doi | 10.1016/j.jbiotec.2016.08.015 | pt |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Improving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems_10.1016j.jbiotec.2016.08.015.pdf | 1.15 MB | Adobe PDF |
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