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http://hdl.handle.net/10773/19270| Title: | Superactivity of alpha-chymotrypsin with biological buffers, TRIS, TES, TAPS, and TAPSO in aqueous solutions |
| Author: | Gupta, Bhupender S. Taha, Mohamed Lee, Ming-Jer |
| Keywords: | BOVINE SERUM-ALBUMIN IONIC LIQUIDS ORGANIC MEDIA WATER STABILITY ENZYME FLUORESCENCE STABILIZATION COMPLEXES PROTEINS |
| Issue Date: | 2014 |
| Publisher: | ROYAL SOC CHEMISTRY |
| Abstract: | Biological buffers are always considered as non-toxic, biocompatible and green compounds. Therefore, we analyzed the catalytic activity of a commercial enzyme alpha-chymotrypsin (alpha-CT) in aqueous solutions of some common biological buffers (TRIS, TES, TAPS, and TAPSO) at pH 8 and T = 25 degrees C. It is found that the increase of the buffer concentration enhanced the catalytic activity of enzyme alpha-CT, and the tendency follows the order of TRIS > TES > TAPS > TAPSO. Especially in the presence of TRIS, the catalytic activity enhanced 5.5 fold. |
| Peer review: | yes |
| URI: | http://hdl.handle.net/10773/19270 |
| DOI: | 10.1039/c4ra09434d |
| ISSN: | 2046-2069 |
| Publisher Version: | 10.1039/c4ra09434d |
| Appears in Collections: | CICECO - Artigos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Superactivity of α-chymotrypsin with biological buffers TRIS TES TAPS and TAPSO in aqueous solutions_10.1039c4ra09434d.pdf | 1.28 MB | Adobe PDF | View/Open |
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