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http://hdl.handle.net/10773/11250
Title: | Evaluation of oxidative DNA lesions in plasma and nuclear abnormalities in erythrocytes of wild fish (Liza aurata) as an integrated approach to genotoxicity assessment |
Author: | Oliveira, M. Ahmad, I. Maria, V. L. Ferreira, C. S. S. Serafim, A. Bebianno, M. J. Pacheco, M. Santos, M. A. |
Keywords: | Liza aurata Genotoxicity 8-Hydroxy-2′-deoxyguanosine Erythrocytic nuclear abnormalities |
Issue Date: | 2010 |
Publisher: | Elsevier |
Abstract: | Genetic lesions (8-hydroxy-2′-deoxyguanosine (8-OHdG) and erythrocytic nuclear abnormalities (ENA) were seasonally quantified in the blood of Liza aurata caught at Ria de Aveiro (Portugal), a multi-contaminated aquatic system. Thus, five critical sites were assessed and compared with a reference site (Torreira). Oxidative DNA damage was found in Gafanha (harbour-water area), Laranjo (metal-contaminated) and Vagos (contaminated with PAHs) in the spring; Rio Novo do Principe (near a former paper-mill effluent) in the autumn; Rio Novo do Principe and Vagos in the winter. ENA were higher than Torreira at VAG (spring and winter). Torreira did not display seasonal variation neither in terms of 8-OHdG or total ENA. A positive correlation between 8-OHdG and ENA was found, suggesting oxidative stress as a mechanism involved in the formation of ENA. This study clearly demonstrates the presence of DNA-damaging substances in Ria de Aveiro and recommends the use of 8-OHdG and ENA as biomarkers of environmental contamination. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/11250 |
DOI: | 10.1016/j.mrgentox.2010.08.003 |
ISSN: | 1383-5718 |
Appears in Collections: | CESAM - Artigos DBio - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Evaluation of oxidative DNA lesions in plasma.pdf | 378.57 kB | Adobe PDF |
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