Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/30790
Title: Macrophage metabolomics reveals differential metabolic responses to subtoxic levels of silver nanoparticles and ionic silver
Author: Carrola, Joana
Bastos, Verónica
Daniel-da-Silva, Ana L.
Gil, Ana M.
Santos, Conceição
Oliveira, Helena
Duarte, Iola F.
Keywords: Macrophages
Silver
Nanoparticles
NMR metabolomics
Cell metabolism
Issue Date: 22-May-2020
Publisher: Wiley-VCH Verlag
Abstract: This study employed NMR metabolomics to characterize macrophage responses to subtoxic concentrations of silver nanoparticles (AgNPs ca. 30 nm) and ionic silver (Ag+), with a view to further elucidate their immunomodulatory activity at the cell metabolism level. Exposure to AgNPs caused RAW 264.7 macrophages to decrease intracellular glucose utilization, possibly due to interference with glycolytic enzymes, and to reprogram the TCA cycle towards anaplerotic fueling and production of anti-inflammatory metabolites (e.g. itaconate and creatine). Moreover, AgNPs-exposed cells were able to control the levels of reactive oxygen/nitrogen species (ROS/RNS), likely through upregulation of glutathione synthesis. On the other hand, macrophages exposed to Ag+ at equivalent subtoxic concentrations showed reduced metabolic activity, lower ability to counterbalance ROS/RNS and alterations in membrane-related lipids. Overall, the metabolomics approach hereby employed provided novel insights into the differential effects of AgNPs and Ag+, which help explain the lower toxic potential of nanosilver than silver ions.
Peer review: yes
URI: http://hdl.handle.net/10773/30790
DOI: 10.1002/ejic.202000095
ISSN: 1434-1948
Publisher Version: https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/ejic.202000095
Appears in Collections:CESAM - Artigos
CICECO - Artigos
DBio - Artigos
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