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http://hdl.handle.net/10773/30790
Título: | Macrophage metabolomics reveals differential metabolic responses to subtoxic levels of silver nanoparticles and ionic silver |
Autor: | Carrola, Joana Bastos, Verónica Daniel-da-Silva, Ana L. Gil, Ana M. Santos, Conceição Oliveira, Helena Duarte, Iola F. |
Palavras-chave: | Macrophages Silver Nanoparticles NMR metabolomics Cell metabolism |
Data: | 22-Mai-2020 |
Editora: | Wiley-VCH Verlag |
Resumo: | This study employed NMR metabolomics to characterize macrophage responses to subtoxic concentrations of silver nanoparticles (AgNPs ca. 30 nm) and ionic silver (Ag+), with a view to further elucidate their immunomodulatory activity at the cell metabolism level. Exposure to AgNPs caused RAW 264.7 macrophages to decrease intracellular glucose utilization, possibly due to interference with glycolytic enzymes, and to reprogram the TCA cycle towards anaplerotic fueling and production of anti-inflammatory metabolites (e.g. itaconate and creatine). Moreover, AgNPs-exposed cells were able to control the levels of reactive oxygen/nitrogen species (ROS/RNS), likely through upregulation of glutathione synthesis. On the other hand, macrophages exposed to Ag+ at equivalent subtoxic concentrations showed reduced metabolic activity, lower ability to counterbalance ROS/RNS and alterations in membrane-related lipids. Overall, the metabolomics approach hereby employed provided novel insights into the differential effects of AgNPs and Ag+, which help explain the lower toxic potential of nanosilver than silver ions. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/30790 |
DOI: | 10.1002/ejic.202000095 |
ISSN: | 1434-1948 |
Versão do Editor: | https://chemistry-europe.onlinelibrary.wiley.com/doi/abs/10.1002/ejic.202000095 |
Aparece nas coleções: | CESAM - Artigos CICECO - Artigos DBio - Artigos DQ - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Carrola et al 2020_EurJIC.pdf | 2.88 MB | Adobe PDF |
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